Composite

Part:BBa_K2259070:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-02)


Rop protein - SynORI Inducible global copy number regulator(All groups)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to mutate the sequence to avoid prohibited restriction sites.


Source

This part is a modified version of wildtype ColE1 Rop protein taken from addgene.

References